Graphene Layer Enables Advance in Super-Resolution Microscopy
September 5, 2019 | University of GöttingenEstimated reading time: 1 minute

Researchers at the University of Göttingen have developed a new method that takes advantage of the unusual properties of graphene to electromagnetically interact with fluorescing (light-emitting) molecules. This method allows scientists to optically measure extremely small distances, in the order of 1 ångström (one ten-billionth of a meter) with high accuracy and reproducibility for the first time. This enabled researchers to optically measure the thickness of lipid bilayers, the stuff that makes the membranes of all living cells.
Researchers from the University of Göttingen led by Professor Enderlein used a single sheet of graphene, just one atom thick (0.34 nm), to modulate the emission of light-emitting (fluorescent) molecules when they came close to the graphene sheet. The excellent optical transparency of graphene and its capability to modulate through space the molecules’ emission made it an extremely sensitive tool for measuring the distance of single molecules from the graphene sheet. The accuracy of this method is so good that even the slightest distance changes of around 1 ångström (this is about the diameter of an atom or half a millionth of a human hair) can be resolved.
The scientists were able to show this by depositing single molecules above a graphene layer. They could then determine their distance by monitoring and evaluating their light emission. This graphene-induced modulation of molecular light emission provides an extremely sensitive and precise “ruler” for determining single molecule positions in space. They used this method to measure the thickness of single lipid bilayers which are constituted of two layers of fatty acid chain molecules and have a total thickness of only a few nanometers (1 billionth of a meter).
“Our method has enormous potential for super-resolution microscopy because it allows us to localise single molecules with nanometre resolution not only laterally (as with earlier methods) but also with similar accuracy along the third direction, which enables true three-dimensional optical imaging on the length scale of macromolecules,” says Arindam Ghosh, the first author of the paper.
“This will be a powerful tool with numerous applications to resolve distances with sub-nanometer accuracy in individual molecules, molecular complexes, or small cellular organelles,” adds Professor Jörg Enderlein, the publication’s corresponding author and head of the Third Institute of Physics (Biophysics) where the work took place.
Suggested Items
Real Time with... IPC APEX EXPO 2025: Schmoll America—Committed to Supporting Customers
03/31/2025 | Real Time with...IPC APEX EXPOKurt Palmer of Schmoll America and Stephan Kunz of Schmoll Maschinen GmbH had a great show, reporting solid attendance and good opportunities, as Schmoll America celebrates its first anniversary. With a booth full of equipment for attendees to see and touch, they showcased unique products like the Pico laser and X-ray machine, and discussed plans for a new facility.
Technica USA and CBT Introducing TiTAN Hybrid at IPC APEX EXPO 2025
03/18/2025 | Technica USAThe wait is over! Technica and CBT are proud to unveil TiTAN Hybrid, a groundbreaking innovation set to redefine the PCB industry. Designed for unmatched performance, efficiency, and adaptability, this cutting-edge laser imaging technology brings the future to you—today.
Teledyne Delivers 100th Infrared Detector for the Space Development Agency's Tracking Layer
03/06/2025 | TeledyneTeledyne Technologies Incorporated, a leading provider of advanced imaging solutions, is proud to announce its continuing pattern of on-time and early deliveries for the Space Development Agency's (SDA) proliferated constellation.
Singular Photonics Emerges from Stealth with Portfolio of SPAD-based Image Sensors
01/23/2025 | PRNewswireSingular Photonics emerged from stealth mode today, launching a new generation of image sensors based on single photon avalanche diodes (SPADs). A spin-out from the University of Edinburgh lab of digital imaging pioneer
Imec, Partners Unveil SWIR Sensor with Lead-free Quantum Dot Photodiodes
12/31/2024 | ImecAt the 2024 IEEE International Electron Devices Meeting (IEDM), imec, a world-leading research and innovation hub in nanoelectronics and digital technologies, and its partners in the Belgian project Q-COMIRSE, present a first of its kind prototype shortwave infrared image sensor with indium arsenide quantum dot photodiodes.