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New Microscope Creates Near-Real-Time Videos of Nanoscale Processes
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The big picture
Atomic force microscopes typically scan samples using an ultrafine probe, or needle, that skims along the surface of a sample, tracing its topography, similarly to how a blind person reads Braille. Samples sit on a movable platform, or scanner, that moves the sample laterally and vertically beneath the probe. Because AFMs scan incredibly small structures, the instruments have to work slowly, line by line, to avoid any sudden movements that could alter the sample or blur the image. Such conventional microscopes typically scan about one to two lines per second.
“If the sample is static, it’s ok to take eight to 10 minutes to get a picture,” Youcef-Toumi says. “But if it’s something that’s changing, then imagine if you start scanning from the top very slowly. By the time you get to the bottom, the sample has changed, and so the information in the image is not correct, since it has been stretched over time.”
To speed up the scanning process, scientists have tried building smaller, more nimble platforms that scan samples more quickly, albeit over a smaller area. Bozchalooi says that such scanners, while speedy, don’t allow scientists to zoom out to see a wider view or study larger features.
“It’s like if you were landing somewhere in the United States and have no clue where you’re landing, and are told wherever you land, you’re only allowed to look a few blocks around and up to a limited height,” Bozchalooi says. “There’s no way you can get a bigger picture.”
Scanning in synchrony
Bozchalooi came up with a design to enable high-speed scanning over both large and small ranges. The main innovation centers on a multiactuated scanner and its control: A sample platform incorporates a smaller, speedier scanner as well as a larger, slower scanner for every direction, which work together as one system to scan a wide 3-D region at high speed.
Other attempts at multiactuated scanners have been stymied, mostly due to the interactions between scanners: The movement of one scanner can affect the precision and motion of the other. Researchers have also found that it’s difficult to control each scanner separately and get them to work with every other component of a microscope. To scan each new sample, Bozchalooi says a scientist would need to make multiple tunings and adjustments to multiple components in the instrument.
To simplify the use of the multiactuated instrument, Bozchalooi developed control algorithms that take into account the effect of one scanner on the other.
“Our controller can move the little scanner in a way that it doesn’t excite the big scanner, because we know what sort of motion triggers this scanner, and vice versa,” Bozchalooi says. “In the end, they’re working in synchrony, so from the perspective of the scientist, this scanner looks like a single, high-speed, large-range scanner that does not add any complexity to the operation of the instrument.”
After optimizing other components on the microscope, such as the optics, instrumentation, and data acquisition systems, the team found that the instrument was able to scan a sample of calcite forward and backward, without any damage to the probe or sample. The microscope scans a sample faster than 2,000 hertz, or 4,000 lines per second — 2,000 times faster than existing commercial AFMs. This translates to about eight to 10 frames per second. Bozchalooi says the instrument has no limit on imaging range and for a maximum probe speed, can scan across hundreds of microns, as well as image features that are several microns high.
“We want to go to real video, which is at least 30 frames per second,” Youcef-Toumi says. “Hopefully we can work on improving the instrument and controls so that we can do video-rate imaging while maintaining its large range and keeping it user-friendly. That would be something great to see.”
This research was supported, in part, by the Center for Clean Water and Clean Energy at MIT and KFUPM, and by National Instruments.
