Glass Microchip for Ultrafast Separation and Purification of DNA Fragments
May 30, 2017 | University of TwenteEstimated reading time: 2 minutes
Researchers of the University of Twente developed a glass microchip for ultrafast separation and purification of DNA fragments. The chip, moreover, is easy to produce and cheap.
The new chip is capable of fractionating DNA fragments within just a few minutes, while conventional approaches take hours. The chip does this in high resolution and also purifies the fragments; it removes the other salts in the DNA sample. Tiny amounts of DNA, like in medical diagnostics or in forensics, will be sufficient. The fragments are typically in the size range of second generation DNA sequencing, the next step after the well-known Human Genome Project.
Moving Snake
UT scientist Burcu Gumuscu and her colleagues achieved this high speed and resolution by inventing a new approach to the common technique of gel electrophoresis. In the conventional approach, the DNA moves in a gel by an applied electric field. Larger fragments will move slower than small ones, and in this way size separation is possible. But the electric field has one direction and the molecules move in a straight line. What about varying the field, the UT scientists thought. If you periodically apply an electric field of different magnitude in the perpendicular direction, the fragments will also respond to this. They don’t just move in a straight line now, but somewhere in between the two field directions. As large fragments respond differently - like a snake moves - to the fields than small ones, fragments can be separated.
By applying electric fields in two directions, larger and smaller fragments move in a different way and exit at different micro channels.
Cheap
For collecting the fragments, the chip has arrays of micro channels on the sides of a square separation chamber of about 1 square centimeter. Next to that, it has a DNA reservoir and electrodes for applying the electric fields. The chip is relatively easy to produce using fundamental cleanroom techniques of the MESA+ NanoLab, and cheap. It is versatile as well, as the type and concentration of gel and the electric fields can be adjusted to the application. Protein separation is one of the other possible application areas.
The research shows that DNA fragments between 0,5 kbp and 10 kpb (1 kbp= 1000 base pairs) can be fractionated within two minutes, at high resolution. These are typically the smaller fragments, essential for the second generation DNA sequencing. Speeding up the separation is also a major step forward in medical applications.
The research has been done in the BIOS Lab-on-a-chip group, part of the MESA+ and MIRA research institutes of the University of Twente.
Suggested Items
Altair Acquires Research in Flight, Forging a New Path for Aerodynamic Analysis
05/03/2024 | AltairAltair a global leader in computational intelligence, announced it has acquired Research in Flight, maker of FlightStream®, which provides computational fluid dynamics (CFD) software with a large footprint in the aerospace and defense sector and a growing presence in marine, energy, turbomachinery, and automotive applications.
Intel Takes Next Step Toward Building Scalable Silicon-Based Quantum Processors
05/02/2024 | BUSINESS WIRENature published an Intel research paper, “Probing single electrons across 300-mm spin qubit wafers,” demonstrating state-of-the-art uniformity, fidelity and measurement statistics of spin qubits.
Argonne, Toyota Collaborate on Cutting-Edge Battery Recycling Process
05/01/2024 | BUSINESS WIREThe U.S. Department of Energy’s (DOE) Argonne National Laboratory has recently launched a collaboration with Toyota Motor North America that could reduce the nation’s reliance on foreign sources of battery materials.
Chinese Smartphone Market Maintains its Recovery Momentum at 6.5% Growth in 1Q24,
04/26/2024 | IDCAccording to preliminary data from the International Data Corporation (IDC) Worldwide Quarterly Mobile Phone Tracker, China smartphone shipments grew 6.5% year over year (YoY) to 69.3 million units in 1Q24.
Boeing Opens Research & Technology Center in Japan
04/23/2024 | BoeingBoeing today opened a Boeing Research & Technology (BR&T) Center in Japan that will focus on innovation to enable the commercial aviation industry meet its goal of net zero carbon emissions by 2050.